Fig 2: mRNA expression of 5-LO (A), LTA4H (B), LTC4S (C), PTGS2 (D), PGES (E), PGFS (F), and PGIS (G) in uterine tissues (endometrium and myometrium) on 4th and 13th day of the estrous cycle, and in pregnancy and anestrus phase. Data were normalized against GAPDH for mRNA expression using GraphPad PRISM (Version 8.3.0). Each bar represents one experimental group with SEM. Statistical differences were analyzed withy two-way analysis (ANOVA) of variance followed by Tukey’s post hoc test. The lowest statistical significance was p < 0.05. Asterisks indicate statistical differences between endometrium and myometrium (* p < 0.05; ** p < 0.01; *** p < 0.001). Different letters indicate statistical differences (p < 0.05) between the experimental reproductive stages throughout endometrium (a, b) and myometrium (A, B), respectively.